Monoclonal Antibody Service
Monoclonal antibody, inogadzirwa neB cell clone imwechete, ine homogeneity yakakura inonangana neantigen epitope chaiyo.Monoclonal antibody ine akawanda mabhenefiti, anosanganisira kuchena kwakanyanya, kunzwa, uye hunhu.MaHybridomas anogadzirwa kuburikidza nekusanganiswa kweB lymphocytes iyo inoburitsa mamwe masoja ekudzivirira chirwere, ane masero emyeloma anogara kwenguva refu.Bioantibody inoshandisa tekinoroji yepamusoro-soro yekusanganisa iyo inoshanda zvakapetwa kamakumi maviri pane yakajairwa nzira dzekusanganisa.Pamusoro pezvo, inoshandisa protein microarray yekuongorora tekinoroji kuona monoclonal masoja ekudzivirira anoratidza hunhu hwakanyanya, hukama, uye hunyanzvi hwekuita kurwisa chaiwo epitopes.
| Zvinhu Zvebasa | Kuedza Zviri Mukati | Nguva Yekutungamira (Vhiki) |
| Antigen kugadzirira | 1. Mutengi anopa antigen2. Bioantibody inogadzirira antigen | / |
| Kudzivirira kwembeva | Kudzivirira kweBALB / c mouse, serum collection uye ELISA kuongorora | 4 |
| Cell fusion uye kuongorora | Kusanganiswa kwembeva splenocytes uye myeloma masero, HAT yekuongorora | 2 |
| Yakagadzika cell line kugadzwa | Subcloning yeakaongororwa akanaka clones | 3 |
| Antibody isotype identification | Kuzivikanwa kwesero line subtypes | 1 |
| Small scale incubation | Serum-isina incubation | 2 |
| Kufukidzwa kukuru uye kucheneswa | 200mL serum-yemahara incubation uye kucheneswa | 1 |
