Prokaryotic Protein Expression
Iyo prokaryotic E. coli expression system inobvumwa nevakawanda seyo inodhura zvakanyanya, ine ruzivo rwakakura, uye inowanzo shandiswa yeprotein kutaura.PaBioantibody, takazvipira kupa vatengi vedu zvizere, zvekumira-imwe masevhisi kubva kugene synthesis kusvika kuprotein kutaura uye kucheneswa.Masevhisi edu anosanganisira emahara codon optimization uye kushandisa yedu proprietary tekinoroji kugadzirisa chero nyaya dzine chekuita nekushomeka uye kusanyungudika kunogona kumuka panguva yese yekutaura uye kucheneswa maitiro.Vatengi vedu vanongoda kupa jini kana amino acid kutevedzana kweprotein uye isu tinogona kuendesa emhando yepamusoro mapuroteni muFAST semavhiki matatu.Pamusoro pezvo, Bioantibody inopa endotoxin kubvisa uye kumaka masevhisi akagadzirirwa kune edu chaiwo zvinodiwa nevatengi.Isu takazvipira kuunza mhedzisiro uye tinovimbisa kusachaja chero muripo kana puroteni yekupedzisira isina kuratidzwa.
| Zvinhu Zvebasa | Yekuedzwa Content | Nguva Yekutungamira (BD) |
| Gene Synthesis | Codon optimization, gene synthesis uye subcloning. | 5-10 |
| Expression Identification uye Solubility Analysis | 1. Shanduko uye incubation, kuonekwa kwekutaura neSDS-PAGE.2. Solubility analysis, SDS-PAGE uye WB kuona | 10 |
| Yakakura incubation uye kucheneswa, puroteni yekupedzisira (kuchena> 85%, 90%, 95%) uye yakajairwa mushumo wekuyedza. | Affinity kucheneswa (Ni column, MBP, GST) |
Kana iyo gene yakasanganiswa mukatiBioantibody, iyo plasmid yakagadzirwa ichaverengerwa mune zvinounzwa.