Yeast Cell Protein Expression
Yeast expression system inzira inoshandiswa zvakanyanya yeeukaryotic protein expression, nekuda kwekureruka kwayo mukurima, kugona, uye nyore kushanda.Pakati pemhando dzakasiyana dzembiriso, Pichia pastoris ndiyo inonyanya kufarirwa yekutaura inomiririra, sezvo ichifambisa zvese zviri zviviri intracellular uye extracellular protein expression.Iyo sisitimu zvakare inogonesa post-shanduro shanduko, senge phosphorylation uye glycosylation, zvichikonzera yakasarudzika eukaryotic kutaura system ine akawanda mabhenefiti.
| Zvinhu Zvebasa | Nguva Yekutungamira (BD) |
| Codon optimization, gene synthesis uye subcloning | 5-10 |
| Positive clone screening | 10-15 |
| Mukero mudiki kutaura | |
| Hukuru (200ML) kutaura uye kucheneswa, zvinounzwa zvinosanganisira mapuroteni akacheneswa uye mushumo wekuyedza |
Kana jini rikagadzirwa muBioantibody, iyo plasmid yakagadzirwa ichaverengerwa mune zvinounzwa.