Insect Cell Protein Expression
Iyo insect cell expression system inowanzoshandiswa eukaryotic expression system yekuratidza maprotein makuru-molecular.Kuenzaniswa nemasero emammalian, zvipembenene sero tsika mamiriro ari nyore uye haadi CO2.Baculovirus imhando yehutachiwana hweDNA hwakapetwa kaviri nemasero ezvipembenene seanogashira.Iine hunhu hwemhando yepamusoro, haiparadzi mhuka dzemuzongoza, uye haina ngozi kuvanhu nezvipfuyo.sf9, inonyanya kushandiswa sesero rekugamuchira, rinowanikwa mu planktonic kana kutevedzera tsika.sf9 yakanyatsokodzera kutaura kwakakura, uye inogona kushandiswa kunotevera kugadzirisa uye kuchinjisa mapuroteni akadai se phosphorylation, glycosylation, uye acylation.Iyo insect cell expression system inogona zvakare kushandiswa kutaura kweakawanda majini, uye inogona zvakare kuratidza huturu mapuroteni senge antimicrobial peptides.
| Zvinhu Zvebasa | Nguva Yekutungamira (BD) |
| Codon optimization, gene synthesis uye subcloning | 5-10 |
| P1 chizvarwa hutachiona incubation uye diki chiyero kutaura | 10-15 |
| P2 chizvarwa chevhairasi incubation, yakakura kutaura uye kucheneswa, kuburitswa kweprotein yakacheneswa uye mushumo wekuyedza |