Chinangwa Kushandiswa
Ichi chigadzirwa chakakodzera kuongororwa kwekiriniki kweserum/plasma/sampuli dzeropa rose kuti zvionekwe masoja ekudzivirira chirwere anorwisa Leishmania.Iyo iri nyore, inokurumidza uye isiri-instrumental bvunzo yekuongororwa kwekala-azar yakakonzerwa neLeishmania.
Test Principle
Ichi chigadzirwa ndeye lateral flow chromatographic immunoassay.The test cassette ine: 1) burgundy colored conjugate pad ine recombinant rK39 antigen conjugated ne colloid gold (Leishmania conjugates) uye tsuro IgG-goridhe conjugates;2) nitrocellulose membrane strip ine maviri ebvunzo mabhendi (M uye G mabhendi) uye control bhendi (C bhendi).
Zvishandiso / zvakapihwa | Huwandu (1 Muedzo/Kit) | Huwandu(5Tests/Kit)
| Huwandu(25Tests/Kit)
|
Test Kit | 1 bvunzo | 5 miedzo | 25 miedzo |
Buffer | 1 bhodhoro | 5 mabhodhoro | 25/2 mabhodhoro |
Drop | 1 chidimbu | 5 pcs | 25 pcs |
Muenzaniso Yekutakura Bag | 1 chidimbu | 5 pcs | 25 pcs |
Disposable Lancet | 1 chidimbu | 5 pcs | 25 pcs |
Mirayiridzo Yekushandisa | 1 chidimbu | 1 chidimbu | 1 chidimbu |
Certificate of Conformity | 1 chidimbu | 1 chidimbu | 1 chidimbu |
Unganidza Serum yemunhu/Plasma/Ropa rose zvakanaka.
Ndapota verenga mirayiridzo zvakanyatsonaka usati waedza.Usati waedza, bvumidza kit yekuyedza, mhinduro yemuenzaniso uye sampuli kuti ienzane netembiricha (15-30 ℃ kana 59-86 madhigirii Fahrenheit).
1.Bvisa chubhu yekubvisa kubva pakiti uye bhokisi rekuedza kubva mubhagi refirimu nekubvarura notch.Zviise pahorizontal ndege.
2.Vhura kadhi rekuongorora aluminium foil bag.Bvisa kadhi rekuedza uye riise rakachinjika patafura.
3.Shandisa pipette inoraswa, tumira donho rimwe (inenge 20μL) ropa remunwe / kana 4μL serum / kana 4μL plasma / kana 4μL ropa rose mumuenzaniso zvakanaka pane test cassette.
4.Vhura buffer tube.Isa madonhwe matatu (angangoita 80 μL) yeassay diluent muyedzo diluent yakatenderedzwa zvakanaka.Tanga kuverenga.
Verenga zvawanikwa pa5-10 mins.Zvabuda mushure memaminitsi gumi hazvisi kushanda.
Negative mhedzisiro
Kana chete mhando yekudzora mutsara C ichioneka uye mitsetse yekuona G uye M isingaratidzi
Positive Result
1.Both quality control line C uye mutsara wekutsvaga M kuonekwa= Leishmania IgM antibody inowanikwa, uye chigumisiro chakanaka kune IgM antibody.
2.Zvose mutsara wehutano hweC uye mutsara wekutsvaga G unooneka= Leishmania IgG antibody inowanikwa uye chigumisiro chakanaka kune IgG antibody.
3.Zvese mutsara wekutonga mhando C uye mitsara yekuona G uye M inooneka=masoja ekudzivirira chirwere anonzi Leishmania IgG neIgM.vanoonekwa, uye mhedzisiro yacho yakanaka kune ese maIgG neIgM masoja ekudzivirira chirwere.
Mhedzisiro isiriyo
Iyo yemhando yekudzora mutsara C haigone kucherechedzwa, mhedzisiro ichave isina kunaka zvisinei nekuti mutsara wekuyedza unoratidza, uye bvunzo inofanira kudzokororwa.
Product Name | Katsi.Aihwa | Size | Muenzaniso | Sherufu Hupenyu | Trans.& Sto.Temp. |
Leishmania IgG/IgM Antibody Test Kit (Immunochromatographic Assay) | B020C-01 | 1test/kiti | Serum/Plasma/Ropa Rakazara | 18 Mwedzi | 2-30 ℃ / 36-86℉ |
B020C-05 | 5 bvunzo/kiti | ||||
B020C-25 | 25 bvunzo/kiti |