Monkeypox Virus Real Time PCR Kit
Product details
Chinangwa Kushandiswa
Inoshandiswa pakuona kweMonkeypox Virus muserum yevanhu kana lesion exudate samples nekushandisa chaiyo nguva PCR masisitimu.
Test Principle
Ichi chigadzirwa ndeye fluorescent probe-based Taqman® chaiyo-nguva PCR assay system.Maprimers chaiwo uye probes akagadzirirwa kuonekwa kweF3L gene yeMonkeypox Virus.Kudzora kwemukati kwakananga kumunhu akachengetedzwa jini tarisisa kuunganidzwa kwemuenzaniso, sampuli kubata uye chaiyo-nguva PCR maitiro kudzivirira nhema-negative mhedzisiro.Iyo kit ndeye yakazara premix lyophilized system, inosanganisira zvinhu zvinodiwa paMonkeypox Virus kuonekwa: nucleic acid amplification enzyme, UDG enzyme, reaction buffer, chaiyo primer uye probe.
Main Contents
Zvikamu zvakapihwa zvakanyorwa patafura.
| Zvikamu | Package | Ingredient |
| Monkeypox VirusLyophilized Premix | 8 bvisa PCR machubhu× 6 homwe | Maprimers, probes, dNTP/dUTP Mix, Mg2+, Taq DNA polymerase, UDG Enzyme |
| MPV Positive Control | 400 μL × 1 chubhu | DNA sequences ine Target gene |
| MPV Negative Control | 400 μL × 1 chubhu | DNA sequences ine chejini remunhu |
| Dissolving Solution | 1 mL × 1 chubhu | Stabilizer |
| Certificate of Conformity | 1 chidimbu | / |
Operation Flow
1. MuenzanisoKuunganidzwa:Muenzaniso unofanirwa kuunganidzwa mumachubhu asina utachiona maererano
neyakajairwa technical specifications.
2. Kugadzirira Reagent (Reagent Preparation Area)
Bvisa zvikamu zvekiti, zvienzane patembiricha yekamuri kuitira kushandiswa kwekumirira.
3. Kugadziridza kweSpecimen (Nzvimbo Yekugadziridza)
3.1 Nucleic acid kubviswa
Zvinokurudzirwa kutora 200μL liquid samples, Positive Control uye Negative Control ye nucleic acid kutorwa, maererano nezvinodiwa zvinoenderana uye maitiro ehutachiwana DNA extraction kits.
3.2 Lyophilized poda kunyungudika uye kuwedzera template
Gadzirira Monkeypox Virus Lyophilized premix zvichienderana nehuwandu hwemasampuli.Sample imwe inoda imwe PCR chubhu ine Lyophilized premix poda.Negative control & positive control inofanirwa kubatwa semasamples maviri.
(1) Wedzera 15μL Dissolving Solution mune yega yega PCR chubhu ine Lyophilized premix, wobva wawedzera 5μL yakatorwa samples/ Negative Control/Positive Control mune yega PCR chubhu zvichiteerana.
(2) Fukidza PCR machubhu zvakasimba, flick PCR machubhu neruoko kusvikira lyophilized poda yanyungudika zvachose uye yasanganiswa, unganidza mvura kusvika kuzasi kwePCR machubhu nekudzika-speed centrifugation pakarepo.
(3) Kana ukashandisa zvakajairika Real-nguva PCR chiridzwa chekuonekwa, wobva waendesa zvakananga PCR machubhu kunzvimbo yekusimudzira;kana ukashandisa BTK-8 kuti uone, saka unoda kuita zvinotevera mashandiro: kuendesa 10 μL mvura kubva kuPCR chubhu kuenda kune reaction chip mutsime weBTK-8.Imwe PCR chubhu inoenderana neimwe tsime pane chip.Panguva yekushanda kwepipetting, simbisa kuti pipette iri 90 degrees vertical.Iyo aerosol barriers pipette mazano anofanirwa kuiswa pakati pegomba nesimba rine mwero uye rega kusundira pipette kana yasvika kune yekutanga gear (kudzivisa mabhubhu).Mushure mekunge matsime azadzwa, tora reaction chip membrane kuvhara matsime ese uye chip chinobva chaendeswa kunzvimbo yekuona amplification.
4. PCR Amplification (Detection Area)
4.1 Isa iyo PCR machubhu / reaction chip mutangi rekuita uye isa mazita ega ega maitiro muhurongwa hunoenderana.
4.2 Zvirongwa zvekuona fluorescence: (1) Monkeypox virus (FAM);(2) Kudzora Kwemukati (CY5).
4.3 Mhanya inotevera bhasikoro protocol
Protocol yeABI7500, Bio-Rad CFX96, SLAN-96S, QuantStudio:
| Matanho | Tembiricha | Nguva | Cycles | |
| 1 | Pre-denaturation | 95℃ | 2 mins | 1 |
| 2 | Denaturation | 95℃ | 10 p | 45 |
| Anealing, kuwedzera, fluorescence kuwana | 60 ℃ | 30 p | ||
Protocol yeBTK-8:
| Matanho | Tembiricha | Nguva | Cycles | |
| 1 | Pre-denaturation | 95℃ | 2 mins | 1 |
| 2 | Denaturation | 95℃ | 5 p | 45 |
| Anealing, kuwedzera, fluorescence kuwana | 60 ℃ | 14 p | ||
5. Ongororo yemhedzisiro (ndapota tarisa kune Instrument User Manual)
Mushure mekuita, mibairo inozochengetwa otomatiki.Dzvanya "Ongorora" kuti uongorore, uye chiridzwa chinozodudzira otomatiki Ct kukosha kwega rega sampuli mumhedzisiro column.Izvo zvisina kunaka uye zvakanaka mhedzisiro yekudzora ichaenderana neinotevera "6. Quality Control ".
6. Quality Control
6.1 Kudzora Kwakashata: Kwete Ct kana Ct> 40 muFAM chiteshi, Ct≤40 muCY5 chiteshi ine yakajairika amplification curve.
6.2 Kudzora Kwakanaka: Ct≤35 muFAM chiteshi ine yakajairika amplification curve, Ct≤40 muCY5 chiteshi ine yakajairika amplification curve.
6.3 Mhedzisiro inoshanda kana zvese zviri pamusoro zvazadzikiswa.Zvikasadaro, mhedzisiro yacho haina basa.
Tsanangudzo yemhedzisiro
Izvi zvinotevera zvinogoneka:
| Ct kukosha kweFAM chiteshi | Ct kukosha kweCY5 chiteshi | Dudziro | |
| 1# | Hapana Ct kana Ct> 40 | ≤40 | Monkeypox virus negative |
| 2# | ≤40 | Chero zvabuda | Monkeypox virus positive |
| 3# | 40～45 | ≤40 | Re-test;kana ichiri 40 ~ 45, taura se 1# |
| 4# | Hapana Ct kana Ct> 40 | Hapana Ct kana Ct> 40 | Hazvina basa |
CHERECHEDZA: Kana mhedzisiro isiriyo ikaitika, sampuli inoda kuunganidzwa uye kuongororwa zvakare.
Order Information
| Product Name | Katsi.Aihwa | Size | Muenzaniso | Sherufu Hupenyu | Trans.& Sto.Temp. |
| Monkeypox Virus Real Time PCR Kit | B001P-01 | 48 bvunzo/kiti | Serum/ Lesion Exudate | 12 Mwedzi | -25～-15℃ |
